FastQCFastQC Report
Thu 18 Nov 2021
HLGLLBGXK_n01_910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGLLBGXK_n01_910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37877288
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGCGTTGATTCAGCTAACGAGCGAGATTATCGCCAATTATTTACTT731390.19309460592849204No Hit
CTCCTACTCTCGCCCTTGATCGACAGAAACCCTCTAGGTAGACGCCTTCG711110.1877404739219978No Hit
ACGCTAGACAGGTCAACCCACGCACCACCAACGGCCATGCACCACCAAAA580430.1532395877973101No Hit
GTCTACACCCCCGCAGGAACTATACAAGACTAGACCAAAGTGAAGCTCTA568460.15007938266329945No Hit
CCTTAAGACACTACTCTCAAAGTATCCGACTCTCCCCGGTCCATCGTAAG564440.14901806063834347No Hit
GAGTAGGAGTATCCAACAGGATTAGAGACCCTGGTAGTTCCTACCTTAAA559330.14766896721856115No Hit
CATCAGAGGCACGCCATTCGACTGAGTGACCTATCAGCTTGTACTTAGGG521140.13758640798147956No Hit
GTCTTAAGGGGGTGGTATGTCCCATCCAAGGCTAAATAACGTGAGGAGAC497650.13138480241774436No Hit
ATCCCAGACGGCGTAGCTGCGCGACATACCGTTTCCGATAATCGCTAACC497650.13138480241774436No Hit
GTATTAGGGATCGTGAGCTGGGTTTAGACCGTCGTGAGCCAGGTTTGTTT482880.12748536801262011No Hit
GCTCTGGGCTGCACGCGTGCTACAATGTTAGGATCAATAGGACTGCGAGC474420.12525183957204117No Hit
GTGGTAGTATAGCCAGTGAACGCGACATGAATCCTGCTCAGTGCTGTGTC463870.1224665292826667No Hit
GTCTTAGATGTGCGAAATTACAGCACGCGCTTATTTATAATTTGGCATTA447490.11814203804665212No Hit
GAATAGGACTAGATAGAAAGTTTGGCTGGGGCGGCACACCTTCGAAAGAG440780.11637052790051917No Hit
TACTACCACAGCGCGACACTACGTTTTTATTAAACAGTTGGGCTCTCCGC435610.1150055938534987No Hit
GTATGAAGTCTTTGTTTTCTTCCGAAAACAAGCTCAATGAGAGCCATCGG432010.11405515621920978No Hit
GCCCAGAGCATCTAAAGGGCATCACGGACCTGTTATTGCTACCCTCTTCC431980.1140472359055907No Hit
CCTCTAGATAACGTGATTTAATCACTTTCTGCTCGTAAGCATCCGGTGAT425740.11239981067282326No Hit
ATCCAATAGCCATTGACATAACCGAAGCTATGTTTTGCCAGCAGAACGCC425180.11225196481860053No Hit
AGATAGATCTACCCCCGATGGCTCTCATTGAGCTTGTTTTCGGAAGAAAA423220.11173450432882101No Hit
CCCTAATACGGGTGAACAATCCGACCCTTAGCAACTTGTACATCACTAGG421770.11135168917056573No Hit
GTACTAATACCCAGGGAAACTGGTCATCAGGCGAACTGCGGCGTTCTGCT417140.1101293207686886No Hit
GGTCTAAGTCAGATGGAATGCTGCGCGATTCAGAGTAAAAGCCTCGTATG411670.10868518358547741No Hit
CCTCTGATGAGGTCAACTTTTCCTCCATAATTCACATCTGGAGAAACAAT404550.1068054291532171No Hit
GGTGTAGACTAGGTGGGAGAGCAATCGATTCTGAAAAACCACTACCCTGT396100.10457454081717783No Hit
GATCTATCTCATGACGAGTGGTGGAATACTTTGACTCATGAGAGAGAAGC389840.102921835375331No Hit
CATTACACCTTCGCAAAAATGTCCACATCCCCCTACTCTCAGTTCAATAG385050.10165722530081879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)