FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n02_dmd-3_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_dmd-3_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48877642
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2799930.5728447374773112No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2144380.4387241102997563No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1618960.33122710788707854Illumina RNA PCR Primer (100% over 31bp)
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT548120.11214125264062452Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)