FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_dmd-3_28_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_dmd-3_28_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29242
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7542.5784830038985023No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2560.8754531153819849No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT780.2667396210929485Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)