FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_dmd-3_26_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_dmd-3_26_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38755034
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC4084021.0538037458566027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2505860.6465895501472144No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC1899390.4901014923635469No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1826230.47122394473966916No Hit
TGTTTTTTTGATCGTCGGACTGTAGAACTCTGAACG1599850.41281088799973703Illumina DpnII expression Sequencing Primer (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1594050.4113143082263842No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1525550.39363918504109685No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1519030.391956822951052No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC1374480.35465844256516454No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1263750.3260866704438964No Hit
TGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1259150.32489972786503035No Hit
CGTGGCTTGAGCGCGCTGCATACTTGGATCGTCGGA989400.25529586685435496No Hit
CTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA948440.24472691728253934No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC879900.22704147285743576No Hit
CGTCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA847040.21856257434840595No Hit
TCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA791380.20420056914412718No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT778250.20081262217445092Illumina RNA PCR Primer (100% over 31bp)
ACGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA744900.19220728847767235No Hit
CGATGCTTGAGCGCGCTGCATACTTGGATCGTCGGA731820.18883224305776639No Hit
TGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC659620.1702024051894781No Hit
CGCTGCATACTTGGATCGTCGGACTGTAGAACTCTG624340.1610990716715666Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGTAGCTTGAGCGCGCTGCATACTTGGATCGTCGGA612760.1581110727447691No Hit
CAGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA597230.15410385138611926No Hit
TGTTTTTTTTGATCGTCGGACTGTAGAACTCTGAAC582760.15037014288259945Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGTGCTTGAGCGCGCTGCATACTTGATCGTCGGACT539230.1391380536525913No Hit
GCGCTGCATACTTGGATCGTCGGACTGTAGAACTCT517430.13351297795274802Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTAGCAGCGATCGTCGGACTGTAGAACTCTGAACG515660.13305626309088003Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT511570.13200091631967087No Hit
CGTGTCTTGAGCGCGCTGCATACTTGGATCGTCGGA489840.12639390278950602No Hit
TGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA486580.12555272174448356No Hit
CGTGATTGAGCGCGCTGCATACTTGGATCGTCGGAC460460.11881295214448787No Hit
GCTGCATACTTGGATCGTCGGACTGTAGAACTCTGA458630.1183407554228955Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGTGACTTGAGCGCGCTGCATACTTGGATCGTCGGA447940.11558240408200905No Hit
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT426100.10994700714234956Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA390650.1007998083552191No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)