FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n02_dmd-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_dmd-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571.2984054669703873No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300.683371298405467No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC240.5466970387243736No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA200.45558086560364464No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA160.36446469248291574No Hit
CGTGGCTTGAGCGCGCTGCATACTTGGATCGTCGGA130.296127562642369No Hit
CGTCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA130.296127562642369No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC120.2733485193621868No Hit
CTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA120.2733485193621868No Hit
CGATGCTTGAGCGCGCTGCATACTTGGATCGTCGGA110.2505694760820046No Hit
CAGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA110.2505694760820046No Hit
TGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC90.2050113895216401No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA90.2050113895216401No Hit
ACGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA80.18223234624145787No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC80.18223234624145787No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC70.15945330296127563No Hit
TGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA70.15945330296127563No Hit
TGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC60.1366742596810934No Hit
TCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA60.1366742596810934No Hit
CGTGACTTGAGCGCGCTGCATACTTGGATCGTCGGA60.1366742596810934No Hit
CGTAGCTTGAGCGCGCTGCATACTTGGATCGTCGGA60.1366742596810934No Hit
CGGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA60.1366742596810934No Hit
GCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA50.11389521640091116No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11389521640091116No Hit
TGTTTTTTTGATCGTCGGACTGTAGAACTCTGAACG50.11389521640091116Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT50.11389521640091116No Hit
CGCCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA50.11389521640091116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)