FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_dmd-3_22_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_dmd-3_22_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48668903
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2754910.5660513860359664No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2128120.43726483828904056No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1614820.33179708200943014Illumina RNA PCR Primer (100% over 31bp)
GCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT546730.1123366187234588Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)