FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n02_WT_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888049
Sequences flagged as poor quality0
Sequence length36
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163471.8407768039826629No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.5805985930956513No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51060.5749682731470899No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32510.36608340305546205No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC27710.3120323315492726No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.26147205841119125No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21000.23647343783957867No Hit
TGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.17724247198071277No Hit
TGTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.16068933133194227No Hit
CCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.15697332016589174No Hit
CTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.14661353146053877No Hit
TGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12690.14289752029448827No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA12210.1374924131438693No Hit
GCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.1350150723665023No Hit
GACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.12780826283234373No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC10920.1229661876765809No Hit
TGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.12206533648481109No Hit
TGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10840.12206533648481109No Hit
ACCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.12116448529304127No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA10130.1140702821578539No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.11215597337534303No Hit
GACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.1100164517948897No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC9630.10843996220929251No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9370.10551219583604057No Hit
TGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.10269703586175988No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)