FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_WT_28_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_28_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31813318
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2911220.9150947411395441No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1346160.42314354007337424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA625980.19676664974084124No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT471380.14817064978887143Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)