FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n02_WT_24_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_24_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80082
Sequences flagged as poor quality0
Sequence length36
%GC17

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11501.4360280712269924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10521.3136535051572138No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8901.1113608551234984No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5110.6380959516495592No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.5444419470043206No Hit
GTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.49574186458879643No Hit
CCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.35338777752803374No Hit
CCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.3309108164131765No Hit
TGTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.3109312954221922No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.3071851352363827No Hit
GCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.3059364151744462No Hit
ACCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.3009415349267001No Hit
CTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.2984440948028271No Hit
TGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.29344921455508105No Hit
GACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.2672260932544142No Hit
TCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.2659773731924777No Hit
TGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.25848505282085865No Hit
TGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.2547388926350491No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.24225169201568392No Hit
TGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.23975425189181088No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.23475937164406485No Hit
GACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.22476961114857275No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.2172772907769536No Hit
ACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.206038810219525No Hit
TGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.20229265003371544No Hit
TGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.20104392997177895No Hit
CAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.20104392997177895No Hit
GCTCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.1960490497240329No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.19480032966209634No Hit
GCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.18730800929047728No Hit
CATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.18356184910466772No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.1823131290427312No Hit
CATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1420.17731824879498514No Hit
GAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1410.17606952873304862No Hit
GCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1748208086711121No Hit
TTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1748208086711121No Hit
GACCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1748208086711121No Hit
CTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.167328488299493No Hit
GGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.15234384755625482No Hit
GCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.15234384755625482No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.1498464074323818No Hit
GCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.1498464074323818No Hit
TCCTCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.14235408706076272No Hit
CAGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.13860792687495316No Hit
GAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.13611048675108015No Hit
GCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.13486176668914363No Hit
TAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.1311156065033341No Hit
TGCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1010.126120726255588No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12362328613171499No Hit
ACCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT990.12362328613171499No Hit
TGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.12237456606977848No Hit
GACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.12112584600784196No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.11363352563622288No Hit
GACATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT890.11113608551234982No Hit
GCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.10988736545041332No Hit
TCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1086386453884768No Hit
ATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.10738992532654029No Hit
GTTGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.10738992532654029No Hit
ACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.10614120526460377No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.10489248520266727No Hit
GATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.10239504507879424No Hit
GACCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10114632501685772No Hit
CCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10114632501685772No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10114632501685772No Hit
GTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10114632501685772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)