FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_WT_24_25_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_24_25_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40648545
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4325751.0641832321427493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1798600.4424758622971621No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC984740.24225713368092264No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT822560.20235902662690633No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT531720.1308091101415807Illumina RNA PCR Primer (100% over 31bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA459890.11313812093397192No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC457010.11242960848906154No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA416680.10250797414766014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)