FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n02_WT_22_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_22_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40777
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12192.989430316109572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1810.4438776761409618No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.3703067905927361No Hit
TGCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAA780.19128430242538685No Hit
GCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAAG640.15695122250288154No Hit
GGCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAA620.15204649679966648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)