FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n02_WT_22_26_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n02_WT_22_26_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36216616
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3689071.0186125617037218No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1546740.4270802109175523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1216180.3358071886119896No Hit
TGCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAA1063230.29357519211623745No Hit
GCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAAG699670.19319033009599793No Hit
GGCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAA651480.17988428294901987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)