FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_dmd-3_28_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_28_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29242
Sequences flagged as poor quality0
Sequence length15
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA6672.2809657342179057No Hit
CAAAAAAAAAAAAAA4111.405512618835921No Hit
CCAAAAAAAAAAAAA2190.7489227822994323No Hit
TGGAATTCTCGGGTG1140.3898502154435401Illumina Small RNA Adapter 2 (100% over 15bp)
CAAGTATGCAGCGCG1020.34881335066000957No Hit
ACAAAAAAAAAAAAA860.2940975309486355No Hit
CTAAAAAAAAAAAAA650.2222830175774571No Hit
TAAAAAAAAAAAAAA650.2222830175774571No Hit
CCCAAAAAAAAAAAA590.20176458518569182No Hit
ACCAAAAAAAAAAAA460.1573079816702004No Hit
GGTGGAATTCTCGGG440.15046850420627864No Hit
GCTGGAATTCTCGGG360.12311059435059162No Hit
GGGGTGGAATTCTCG330.11285137815470898No Hit
GGGTGGAATTCTCGG330.11285137815470898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)