FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n01_dmd-3_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209102
Sequences flagged as poor quality0
Sequence length15
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA76573.6618492410402577No Hit
CAAAAAAAAAAAAAA35551.7001272106436094No Hit
CCAAAAAAAAAAAAA15160.7250050214727741No Hit
CAAGTATGCAGCGCG11000.5260590525198229No Hit
TAAAAAAAAAAAAAA5810.27785482683092466No Hit
ACAAAAAAAAAAAAA4650.22237950856519784No Hit
CTAAAAAAAAAAAAA4160.1989459689529512No Hit
ACCAAAAAAAAAAAA3580.1712083098200878No Hit
CCCAAAAAAAAAAAA3280.15686124475136537No Hit
TGCAACTCCAGTCTT3160.15112241872387638No Hit
TGGAATTCTCGGGTG2440.11668946255894252Illumina Small RNA Adapter 2 (100% over 15bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)