FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_dmd-3_26_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_26_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38755034
Sequences flagged as poor quality0
Sequence length15
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG547309014.122268606447358No Hit
CAAAAAAAAAAAAAA12143623.1334303564280193No Hit
AAAAAAAAAAAAAAA11863273.0610913668660436No Hit
AAGTATGCAGCGCGC7735881.9960968167386979No Hit
CCAAAAAAAAAAAAA3483840.8989387030340369No Hit
TAAAAAAAAAAAAAA2392320.6172927109288563No Hit
CCAAGTATGCAGCGC2150130.5548001841515608No Hit
AAAAAAACATGGAAT1855090.47867071926707644No Hit
AAAGTATGCAGCGCG1501470.38742579867172866No Hit
ATAGTATGCAGCGCG1387320.3579715605461732No Hit
GAAAAAAAAAAAAAA1359550.35080603980375813No Hit
ACAGTATGCAGCGCG1017110.2624459057370457No Hit
CAAGTATGCAGCGCC970200.2503416717425664No Hit
TGGAATTCTCGGGTG947520.24448952876676613Illumina Small RNA Adapter 2 (100% over 15bp)
CAAGTATGCAGCGCA907190.2340831387220561No Hit
TAAGTATGCAGCGCG878840.22676796000230576No Hit
CAAGTATGCAGCGCT827240.21345356063937398No Hit
CCCAAAAAAAAAAAA794950.20512173979772538No Hit
CAAGTATGCAGCCGC766840.1978684885168724No Hit
CAAGTATGCAGCGTG765030.19740145241518817No Hit
AAAAAAAACATGGAA685200.17680283805195476No Hit
ACAAAAAAAAAAAAA682260.17604422692546212No Hit
TCAAAAAAAAAAAAA652910.16847101721030613No Hit
TGCAACTCCAGTCTT652590.16838844729177635No Hit
GCTGGAATTCTCGGG637730.1645541067000483No Hit
CCAGTATGCAGCGCG626560.16167190048136715No Hit
CAAGTATGCAGCGGC613510.15830459599132335No Hit
CTAAAAAAAAAAAAA592910.15298915748596686No Hit
GCTGCTACTTGGAAT588100.15174802839806567No Hit
GAAGTATGCAGCGCG582490.15030047451383993No Hit
CAAGTATGCAGCTGG540700.1395173592158376No Hit
ACCAAAAAAAAAAAA503670.1299624714559662No Hit
AGAGTATGCAGCGCG497590.12839364300389983No Hit
TCCAAAAAAAAAAAA489830.12639132247955195No Hit
GCAAAAAAAAAAAAA472790.1219944743178396No Hit
ACAAGTATGCAGCGC471520.12166677495367441No Hit
CTAGTATGCAGCGCG467210.12055466136347602No Hit
TTAGTATGCAGCGCG442390.11415033205750767No Hit
CAAGTATGCAGCGAG419660.10828528753193715No Hit
AATGTATGCAGCGCG418130.10789050010896649No Hit
AAAGAAACATGGAAT400980.1034652685377595No Hit
CAAGTATGCAGCGGG387750.10005151846854268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)