FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n01_dmd-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4390
Sequences flagged as poor quality0
Sequence length15
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG53112.095671981776764No Hit
AAAAAAAAAAAAAAA1944.419134396355353No Hit
CAAAAAAAAAAAAAA1192.7107061503416854No Hit
CCAAAAAAAAAAAAA400.9111617312072893No Hit
TAAAAAAAAAAAAAA280.6378132118451025No Hit
AAGTATGCAGCGCGC260.592255125284738No Hit
CAAGTATGCAGCCGC150.3416856492027335No Hit
GAAAAAAAAAAAAAA140.31890660592255127No Hit
CCCAAAAAAAAAAAA130.296127562642369No Hit
CTAAAAAAAAAAAAA100.22779043280182232No Hit
ACAAAAAAAAAAAAA100.22779043280182232No Hit
ACCAAAAAAAAAAAA90.2050113895216401No Hit
CAAGTATGCAGCGGC80.18223234624145787No Hit
CAAGTATGCAGCGCA80.18223234624145787No Hit
CCAAGTATGCAGCGC70.15945330296127563No Hit
CAAGTATGCAGCGCC70.15945330296127563No Hit
AAAGTATGCAGCGCG60.1366742596810934No Hit
ATAAAAAAAAAAAAA60.1366742596810934No Hit
AAAAAAACATGGAAT60.1366742596810934No Hit
GAAGTATGCAGCGCG50.11389521640091116No Hit
CAAGTATGCAGCGAG50.11389521640091116No Hit
CAAGTATGCAGCTCG50.11389521640091116No Hit
CACAAAAAAAAAAAA50.11389521640091116No Hit
TCAAAAAAAAAAAAA50.11389521640091116No Hit
CTAGTATGCAGCGCG50.11389521640091116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)