FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n01_dmd-3_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32015712
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG350181010.937785797173587No Hit
CAAAAAAAAAAAAAA4210841.3152417163172883No Hit
AAAAAAAAAAAAAAA3558461.1114730167487763No Hit
AAGTATGCAGCGCGC2148660.6711267267771525No Hit
CCAAAAAAAAAAAAA1583400.4945696662938497No Hit
CCAAGTATGCAGCGC985600.3078488462165077No Hit
TAAAAAAAAAAAAAA830300.2593414133660373No Hit
CAAGTATGCAGCCGC770540.2406755782910591No Hit
CAAGTATGCAGCGCC732020.22864398580297074No Hit
CAAGTATGCAGCGGC527200.16466914744860273No Hit
AAAGTATGCAGCGCG516310.1612676925629516No Hit
CAAGTATGCAGCGCA484950.15147250200151727No Hit
GAAAAAAAAAAAAAA447320.13971889802107165No Hit
CAAGTATGCAGGCGC357790.11175450353876247No Hit
CCCAAAAAAAAAAAA347170.10843738224531754No Hit
ACAAAAAAAAAAAAA344710.10766900951632748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)