FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_dmd-3_22_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_dmd-3_22_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48668903
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAA2296260.47181256581846526No Hit
TGGAATTCTCGGGTG1891200.3885848834521707Illumina Small RNA Adapter 2 (100% over 15bp)
AAAAAAAAAAAAAAA1747600.3590793899751552No Hit
CCAAAAAAAAAAAAA955400.19630604782688446No Hit
CAAGTATGCAGCGCG754150.15495520825690276No Hit
GCTGGAATTCTCGGG750740.1542545555218288No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)