FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n01_WT_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_WT_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888049
Sequences flagged as poor quality0
Sequence length15
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA12439314.007447787227957No Hit
CAAAAAAAAAAAAAA9765010.996014859540407No Hit
CAAGTATGCAGCGCG713408.033340502607402No Hit
CCAAAAAAAAAAAAA266102.9964562766243756No Hit
TAAAAAAAAAAAAAA167471.8858193635711542No Hit
ACAAAAAAAAAAAAA79810.8987116701893701No Hit
GAAAAAAAAAAAAAA75630.8516421954193968No Hit
AAGTATGCAGCGCGC66460.7483821275627809No Hit
CTAAAAAAAAAAAAA59400.668882009889094No Hit
CCCAAAAAAAAAAAA59060.6650533923240722No Hit
ACCAAAAAAAAAAAA56120.6319471110265312No Hit
TCAAAAAAAAAAAAA48850.5500822589744485No Hit
TCCAAAAAAAAAAAA35240.39682494997460727No Hit
GCAAAAAAAAAAAAA32690.3681103182369441No Hit
GCCAAAAAAAAAAAA27610.31090626755956036No Hit
CGAAAAAAAAAAAAA22720.25584173846262986No Hit
AAAGTATGCAGCGCG18790.2115874236669373No Hit
CAAGTATGCAGCGCC18700.21057396607619627No Hit
CCAAGTATGCAGCGC18640.2098983276823689No Hit
AAAAAAACATGGAAT18190.20483103972866362No Hit
ATAAAAAAAAAAAAA17020.19165609104902995No Hit
ATCAAAAAAAAAAAA16430.18501231350972752No Hit
CCCCAAAAAAAAAAA16140.1817467279395619No Hit
CTCAAAAAAAAAAAA14670.1651935872907914No Hit
ATAGTATGCAGCGCG12730.14334794589037317No Hit
CCAGTATGCAGCGCG12630.1422218819006609No Hit
ACCCAAAAAAAAAAA11230.126456986044689No Hit
CCTAAAAAAAAAAAA10980.12364182607040829No Hit
ATCCAAAAAAAAAAA10730.12082666609612759No Hit
ACTAAAAAAAAAAAA10560.1189123573136167No Hit
TGGAATTCTCGGGTG9830.11069209018871706Illumina Small RNA Adapter 2 (100% over 15bp)
CAAGTATGCAGCGCT9770.1100164517948897No Hit
ACAGTATGCAGCGCG9390.10573740863398302No Hit
CACAAAAAAAAAAAA9200.1035978870535297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)