FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_WT_28_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_WT_28_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31813318
Sequences flagged as poor quality0
Sequence length15
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA2468040.7757883035023257No Hit
CAAAAAAAAAAAAAA2241390.7045445558366468No Hit
CCAAAAAAAAAAAAA792650.2491566582272242No Hit
TGCAACTCCAGTCTT748450.23526310584768306No Hit
CAAGTATGCAGCGCG594980.18702230304930784No Hit
TGGAATTCTCGGGTG584260.18365264509662274Illumina Small RNA Adapter 2 (100% over 15bp)
TAAAAAAAAAAAAAA403870.12694997736482563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)