FastQCFastQC Report
Thu 20 Oct 2022
HLGK3BGXM_n01_WT_24_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_WT_24_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80082
Sequences flagged as poor quality0
Sequence length15
%GC17

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA1738121.704003396518566No Hit
CAAAAAAAAAAAAAA1414717.665642716215878No Hit
CCAAAAAAAAAAAAA36664.577807747059264No Hit
TAAAAAAAAAAAAAA24213.0231512699483027No Hit
GAAAAAAAAAAAAAA11851.4797332733947703No Hit
CAAGTATGCAGCGCG10901.3611048675108013No Hit
ACAAAAAAAAAAAAA10591.3223945455907695No Hit
ACCAAAAAAAAAAAA8071.0077170899827677No Hit
CCCAAAAAAAAAAAA7620.9515246871956244No Hit
TCAAAAAAAAAAAAA7520.9390374865762594No Hit
TCCAAAAAAAAAAAA6260.7816987587722585No Hit
GCAAAAAAAAAAAAA5550.6930396343747659No Hit
CTAAAAAAAAAAAAA5470.6830498738792737No Hit
GCCAAAAAAAAAAAA3710.46327514297844713No Hit
CGAAAAAAAAAAAAA3490.43580330161584374No Hit
CCCCAAAAAAAAAAA2210.2759671336879698No Hit
ATAAAAAAAAAAAAA1890.23600809170600134No Hit
ATCAAAAAAAAAAAA1880.23475937164406485No Hit
ACCCAAAAAAAAAAA1610.20104392997177895No Hit
CTCAAAAAAAAAAAA1510.18855672935241377No Hit
AACAAAAAAAAAAAA1350.16857720836142953No Hit
AGCAAAAAAAAAAAA1270.15858744786593743No Hit
ATCCAAAAAAAAAAA1230.15359256761819134No Hit
CACAAAAAAAAAAAA1180.14734896730850877No Hit
CGCAAAAAAAAAAAA1140.14235408706076272No Hit
AGAAAAAAAAAAAAA1130.14110536699882623No Hit
ACTAAAAAAAAAAAA1080.13486176668914363No Hit
TTCAAAAAAAAAAAA1040.12986688644139757No Hit
CTCCAAAAAAAAAAA1020.12736944631752456No Hit
CCTAAAAAAAAAAAA1010.126120726255588No Hit
CACCAAAAAAAAAAA970.12112584600784196No Hit
TGCAAAAAAAAAAAA950.11862840588396893No Hit
TTCCAAAAAAAAAAA950.11862840588396893No Hit
GTAAAAAAAAAAAAA940.11737968582203243No Hit
TCCCAAAAAAAAAAA910.11363352563622288No Hit
TACAAAAAAAAAAAA890.11113608551234982No Hit
CCGAAAAAAAAAAAA880.10988736545041332No Hit
AACCAAAAAAAAAAA870.1086386453884768No Hit
AAGTATGCAGCGCGC840.10489248520266727No Hit
ACGAAAAAAAAAAAA830.10364376514073075No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)