FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_WT_24_25_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_WT_24_25_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40648545
Sequences flagged as poor quality0
Sequence length15
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG17828344.38597248683809No Hit
AAAAAAAAAAAAAAA9743612.397037827553237No Hit
CAAAAAAAAAAAAAA6838951.6824587448333022No Hit
AAGTATGCAGCGCGC2472160.6081792103505795No Hit
CCAAAAAAAAAAAAA2432790.5984937468241482No Hit
TAAAAAAAAAAAAAA1697510.4176065834582763No Hit
GAAAAAAAAAAAAAA861180.21185998170414216No Hit
CCAAGTATGCAGCGC696030.171231221191312No Hit
ACAAAAAAAAAAAAA676980.1665447065817485No Hit
TGGAATTCTCGGGTG669780.16477342546947252Illumina Small RNA Adapter 2 (100% over 15bp)
TGCAACTCCAGTCTT664370.1634425045226096No Hit
CCCAAAAAAAAAAAA610210.1501185343780448No Hit
TTTTTTTCGAAGTTT597640.14702617276952964No Hit
ACTGCTTGAGCCTAG459820.11312090014538037No Hit
TCAAAAAAAAAAAAA420120.1033542529013031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)