FastQCFastQC Report
Fri 28 Oct 2022
HLGK3BGXM_n01_WT_22_26_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLGK3BGXM_n01_WT_22_26_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36216616
Sequences flagged as poor quality0
Sequence length15
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAACTCCAGTCTT7139211.9712526427096335No Hit
CAAAAAAAAAAAAAA6914891.9093142219582304No Hit
AAAAAAAAAAAAAAA5911431.6322425043797575No Hit
CCAAAAAAAAAAAAA2206920.609366706154987No Hit
TAAAAAAAAAAAAAA1067310.29470174684459755No Hit
GAAAAAAAAAAAAAA733360.20249269009561796No Hit
CAAGTATGCAGCGCG480420.1326518192643951No Hit
CCCAAAAAAAAAAAA456800.12612995095952642No Hit
ACAAAAAAAAAAAAA392700.10843089260465417No Hit
GCAACTCCAGTCTTT389780.10762463284808277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)