FastQCFastQC Report
Sat 23 Apr 2022
HLG55AFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLG55AFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105099672
Sequences flagged as poor quality0
Sequence length10
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTATAC9982484994.981123252221No Hit
GGGGGGGGGG27670022.632740851941003No Hit
GAAGTATACA3145690.29930540601496836No Hit
CAAGTATACA1898060.18059618682730047No Hit
CGAGTATACA1439970.1370099423335974No Hit
CGAAGTATAA1051290.10002790493960818No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)