FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12333135
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23000.031.05068844
TATGCCG24850.028.46127746
GTGTGCG27100.028.1340548
GCCAGTA38700.024.874861
ATGCCGT30000.023.92043147
GTATGCC30300.023.91132245
TCGTATG31200.022.9999543
GTGCGAG33400.022.62093410
ACCCGTA34050.021.88514532
TGCGAGT35350.021.37310211
CGGGCGC17250.020.39970615
TGTGCGA37150.020.3375269
TGTGTGC39100.020.2054277
CTCGTAT36400.019.90347942
GCGAGTC38850.019.80280312
CGCGTAT13400.019.5693421
CCCGTAA38650.019.10192333
AAGGCGC38950.018.9547238
CCCTCGC39050.018.81829366
AGGCGCA39750.018.57324239
Produced by FastQC (version 0.11.2)