FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11004324
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG138480.12584144196408611No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG125140.11371893448429908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19400.033.4335346
GTGTGCG34400.032.6935168
CGTATGC20200.031.25602144
GCCAGTA37350.027.806441
GTGCGAG40350.027.7876710
GTATGCC23000.027.3004445
TGCGAGT42800.026.35823211
TGTGTGC44850.025.768247
TGTGCGA44350.025.5143579
ATGCCGT26500.025.1268247
TCGTATG25050.024.92854543
GCGAGTC47900.023.76789512
ACCCGTA48150.023.50129532
CTGACAT53950.023.1519011
CCCGTAA51050.022.23383733
ATGTGTG54600.021.925386
ACATGTG54550.021.69284
CTCGTAT28650.021.67534842
AAGGCGC52200.021.47984338
AGGCGCA54150.020.89746539
Produced by FastQC (version 0.11.2)