FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10741660
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG108930.10140890700320061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19700.029.5970946
CGTATGC20000.028.98076244
GTGTGCG27400.025.8112858
GTATGCC23350.025.1183245
GTGCGAG31100.023.18452810
ATGCCGT26700.022.225147
GCCAGTA37300.022.0158311
TCGTATG27250.021.52280243
TGCGAGT34750.020.6500411
TGTGCGA35400.020.2706839
CTCGTAT28350.020.07905442
TGTGTGC37600.019.9102257
GCGAGTC37200.019.29002412
ACCCGTA37300.018.86816632
CTGACAT45850.018.0608791
ACGTCTG34250.017.2244715
TAAGGCG41900.017.12622637
TGGCGGG41800.017.0851457
CCCGTAA41000.017.08128433
GCACACG35900.017.00948511
Produced by FastQC (version 0.11.2)