FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11824300
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125660.10627267576093301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25200.029.57273344
TATGCCG25200.029.57273346
GTATGCC29200.025.52167345
ATGCCGT30600.024.80490947
CGCGCGT19350.024.7847731
TCGTATG32300.023.28457643
TCGGGCG21650.021.511927
CTCGTAT36000.021.08292442
GGCGGGC21450.020.58718713
CGGGCGC21650.019.91895115
CGCGCCT32350.019.304361
TCGCGCG13900.019.11130313
CGCGTAT13800.019.00141
CGGCAAT32550.018.9737471
TGCCGTC40000.018.88934348
GCGGGCG23100.018.81797614
ATCGGGC25700.018.6590946
TATCTCG40200.018.53695339
CACTATA43600.017.17039331
CTATAAT46150.016.67017633
Produced by FastQC (version 0.11.2)