FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10870548
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT21350.032.3216131
CGTATGC17950.025.56325344
TATGCCG18300.025.45127946
TCGGGCG22550.024.1723847
CGGGCGC22750.022.74682615
GGCGGGC22750.022.44353513
GCGGGCG23350.022.16232514
CGCGTAT12900.021.9323441
CGGGCAT21900.021.8993681
CGCGCCT29850.021.4995561
ATCGGGC26600.020.3625876
GGGCGGC25350.020.2778199
GTATGCC24100.020.04185545
TCGTATG22950.019.99327543
GCCAGTA33700.019.5552941
CGCGGCT25250.019.4038471
ATGCCGT23850.019.23934247
CGGCAAT36700.018.7088851
CGCGGAT18100.018.6813551
GCGTGCG36750.017.930774
Produced by FastQC (version 0.11.2)