FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11329818
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21900.031.03463644
CGCGCGT26650.030.1672021
TATGCCG24200.028.65531546
GTATGCC27000.025.93921545
ATGCCGT27450.025.38818447
TCGGGCG27150.023.3803757
CGCGTAT15050.023.1558721
TCGTATG29850.022.76852643
CGGCAAT39550.021.7234671
GGCGGGC29950.020.9643713
ATCGGGC30750.020.6433416
GCCAGTA40550.020.3368341
CGGGCAT26950.020.229011
GCGGGCG31450.019.96448114
CGCGCCT39950.019.9513131
CGGGCGC31950.019.4360915
GGGCGGC32500.019.0009389
CTCGTAT36750.018.77524842
CGCTATG22900.018.530829
GCGGTGT50500.017.901371
Produced by FastQC (version 0.11.2)