FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12292158
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG163560.1330604439025271No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG153820.125136692841078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19800.031.01586546
TATGCCG19750.030.39539148
GTGTGCG41450.025.8012988
GTATGCC24150.025.57191547
TCGTATG24800.024.90178345
GCCAGTA50550.024.0254651
ATGCCGT27050.023.21300949
CTCGTAT26250.022.2119444
GTGCGAG48400.022.16790810
AGTCACG30400.021.44915828
GCACACG32750.021.38426811
ACGTGAT29850.021.26552632
TGTGTGC53300.020.5179827
TGCGAGT52300.020.44888711
GTCACGT32600.020.00166729
CACACGT35750.019.68628712
ACGTCTG35950.019.4807215
TCACGTG33100.019.38683930
CTGACAT61500.019.355051
CACGTGA33700.019.24563831
Produced by FastQC (version 0.11.2)