FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14740828
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG158480.1075109213675107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC31600.033.84575344
TATGCCG32150.033.26640746
ATGCCGT36250.030.07489847
GTATGCC38800.028.00940945
TCGTATG39250.027.3361343
GCCAGTA51500.024.7218571
GTGTGCG33800.024.4963028
CTCGTAT44900.024.12654942
GCCGTCT49200.022.0187549
AGTCACG49800.021.96107528
ACGTCTG52850.020.95431115
GCACACG52650.020.90285711
GTGCGAG39850.020.5179910
ACGACAT53300.020.45362535
GTCACGA53700.020.23764229
GACGACA55700.019.5105834
CACACGT57100.019.15305112
CATCTCG56500.018.9900839
TCTCGTA59200.018.47351341
TGCGAGT44600.018.33271811
Produced by FastQC (version 0.11.2)