FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10737293
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG124740.11617453300380273No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG114660.10678669195299038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19400.031.29966244
TATGCCG19200.031.26631546
GTGTGCG31700.028.2957088
CACCTAG23050.025.89295831
GTATGCC23750.025.56688145
GCCAGTA39150.025.382711
TCGTATG24550.024.73305543
GTGCGAG36750.024.50178310
ATGCCGT24950.024.19870847
ACCTAGC25850.024.02251232
TGCGAGT38000.023.60490611
TGTGCGA41300.021.8856649
TGTGTGC42500.021.7545597
GCGAGTC42650.021.11222512
CGCGTAT13450.021.0362741
TCACCTA28800.020.96351830
ACCCGTA42900.020.34556232
CTGACAT50850.019.9495741
CTCGTAT31000.019.69818142
ATGTGTG51450.019.3788056
Produced by FastQC (version 0.11.2)