FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320635
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16400.029.45192144
TATGCCG17800.027.71680646
ATGCCGT20850.023.00042347
GTATGCC21850.022.42146745
GTGTGCG18200.021.4196918
TCGTATG23200.020.81890543
GCCAGTA30300.019.2439631
CTCGTAT26500.018.61672642
CGGAATA26750.018.3136734
TATCTCG27250.017.59792539
CGGGCGC13350.017.57269915
CGCGTAT9350.016.9744871
CGCTATG28750.016.92013229
GCCGTCT29700.016.84365749
ACGTCTG31000.016.6932315
GGCTGCC30450.016.31522443
GTGCGAG24300.016.18505110
GCACACG31300.016.09251811
GGCGGGC15050.016.04620613
ACCCGTA24750.016.02982732
Produced by FastQC (version 0.11.2)