FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9095709
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184410.2027439532201393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18700.030.9953546
CGTATGC20100.030.03798944
CGCGCGT11450.025.3122831
ATGCCGT23600.025.29068447
TCGTATG23850.025.0251743
GTATGCC25300.023.59143845
CGCGCCT21200.022.7850341
CTCGTAT26900.021.80274442
CGGGCAT14700.020.8896031
TGCCGTC30200.019.4208648
TCGGGCG12000.018.6868927
CGGCAAT23450.018.6860891
CGCGTAT9700.018.1407781
GCGCCTG24450.017.4978072
CGGGCGC11650.017.47191815
GCCGTCT35000.017.34905649
CGCGGAT12550.017.3202571
GCGGGCG12000.017.24981914
GGCGGGC13000.016.98443613
GCGCGTT7200.016.7723161
Produced by FastQC (version 0.11.2)