FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11042222
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG119190.10794023159469172RNA PCR Primer, Index 35 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25050.034.7064546
CGTATGC25450.034.29668444
CGCGCGT25350.031.713241
GTATGCC31600.027.9492645
ATGCCGT32000.027.59989447
TCGTATG32450.027.11034443
TCGGGCG29000.024.0302877
CGCGTAT17450.023.7273061
CTCGTAT36700.023.12472742
GGCGGGC28650.023.11980813
CGGCAAT38150.022.7008321
TATCTCG37950.022.54526539
ATCGGGC32550.021.5156566
GCGGGCG30850.021.4710714
CGCGCCT37400.020.9419381
CGGGCGC31850.020.6886215
GGGCGGC33000.020.594919
GCCAGTA36100.020.1668421
CGCGGCT27500.019.698341
TGCCGTC45500.019.25926648
Produced by FastQC (version 0.11.2)