FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6984391
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183410.26259984585628154No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG103670.14843097988070827RNA PCR Primer, Index 34 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21100.043.16796544
TATGCCG21750.041.56033346
GTATGCC25350.035.93047345
ATGCCGT25750.034.97037547
TCGTATG26300.034.10640343
CTCGTAT28650.030.46569842
TGCCGTC30200.030.1599748
GCCGTCT34550.026.4625549
TCTCGTA35100.024.867341
CGGGCGC9400.023.85565815
TCGGGCG9300.023.7408717
GCGGGCG9450.023.36453614
CATCTCG37650.023.27469339
GGCATCT38800.023.207137
CGCGCGT8700.022.209561
GGCGGGC9850.022.06547713
CGCGTAT8350.019.8347151
ATGGCAT48700.019.62246735
CGGGCAT10850.019.398641
CGCGCCT19450.019.336511
Produced by FastQC (version 0.11.2)