FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557716
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163130.15451258586610966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG115250.10916186796462418RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22700.035.1084146
CGTATGC23850.033.4155544
GTATGCC27700.028.52205345
CGCGCGT17700.027.8778841
TCGTATG29400.027.34117743
CCAGGCG30500.025.90252933
ATGCCGT31800.025.17015647
CGCGTAT14950.024.927551
TCGGGCG21700.024.8006557
CAGGCGA33900.023.20319734
CTCGTAT33650.021.83719842
ATCGGGC26050.021.7189356
GGCGGGC23000.021.59957913
GCGGGCG22900.021.24194314
CGGGCAT20100.020.600711
CGGGCGC23650.020.56840715
CGGCAAT34450.020.4332731
TGCCGTC39650.020.36083848
GGGCGGC25500.019.617179
AGGCGAT40250.019.45639435
Produced by FastQC (version 0.11.2)