FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12433419
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20150.026.02618644
TATGCCG20950.024.70257646
GTATGCC24350.021.11165845
GTGTGCG27050.020.150698
TCGTATG27400.019.64234543
ATGCCGT29950.018.20092647
GCCAGTA39250.017.405021
GGCGGGC23950.016.70975313
CTCGTAT32450.016.47900842
CGGGCGC23450.016.47748815
GTGCGAG33900.016.1813610
GCACACG35850.015.97483811
ACGTCTG37200.015.30230415
TGTGCGA37250.014.9109969
TAGCCCG27400.014.85737234
ACCCGTA36450.014.67043632
CACACGT39500.014.49868312
GCGAGTC38000.014.25389812
CCCTCGC38550.014.05092966
TGTGTGC42050.014.0290577
Produced by FastQC (version 0.11.2)