FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17432980
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG182470.10466942542238905RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36000.035.74639544
TATGCCG36050.035.60080746
GTATGCC43750.029.41392545
TCGTATG46050.028.39417643
ATGCCGT46050.027.86982547
CACGATA49700.026.03099834
GCCAGTA57350.024.9071481
CTCGTAT51750.024.86641542
GTGTGCG45250.024.5496068
TATCTCG53950.023.7885939
GCCGTCT57150.022.57752249
GCACACG62400.021.23055511
GTGCGAG53600.020.72565710
ACGATAT63450.020.71586635
ACGTCTG63550.020.52064115
CACACGT67500.019.47319212
ACCCGTA59950.018.64524332
TGCGAGT60300.018.5943411
CAGTAGT75950.018.3967843
AGTAGTC73650.018.2686544
Produced by FastQC (version 0.11.2)