FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9577545
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17150.031.18093744
TATGCCG17050.030.35192146
GTATGCC19600.027.10715745
GTGTGCG19250.024.7319628
ATGCCGT21500.024.7116447
TCGTATG23250.022.85138743
CTCGTAT26100.019.5628242
GCCAGTA29650.019.4337431
ACGTCTG29500.019.29621715
GTGCGAG24800.019.1974910
GCACACG29400.019.12736111
CGGGCGC14150.019.0172815
CACACGT32250.017.75796312
GCCGTCT29800.017.7131949
TGCGAGT27000.017.50538311
TGTGCGA27600.017.4997359
ACCCGTA26850.017.47459632
GGCGGGC15800.017.46799913
AGGCGCA29800.016.0920439
CGCTATG28400.016.03547129
Produced by FastQC (version 0.11.2)