FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9843750
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17800.029.46062946
CGTATGC18450.028.42286744
GTATGCC21200.025.06128345
ATGCCGT22700.023.2532747
TCGTATG24050.022.09099643
GTGTGCG21050.021.1418448
CTCGTAT27950.018.63794942
GCCGTCT30100.018.33865749
CGCGGGG2851.6370905E-1118.1598971
GTGCGAG25500.017.5881210
GCCAGTA28900.017.5503791
TGCGAGT26950.016.64173511
GCACACG33550.016.55569511
ACGTCTG34150.016.36550915
TGTGCGA27900.016.3222249
GCGAGTC28550.016.07161712
ACCCGTA27800.016.00856232
TGCCGTC35050.015.4533848
AGGCGCA29450.015.11149539
CCCTCGC29500.014.97000266
Produced by FastQC (version 0.11.2)