FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425218
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64061.506521360807868No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG62631.4728915520979828RNA PCR Primer, Index 28 (100% over 43bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7200.169324910986835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7150.062.24942446
CGTATGC7200.061.81713544
GTATGCC7350.060.5555645
ATGCCGT7900.056.3396747
TCGTATG8000.055.19764743
CTCGTAT7750.054.7525142
TGCCGTC8150.054.61145448
TCTCGTA7900.053.71290641
GCCGTCT8400.052.9861249
CTGCTTG8350.052.4769957
ACGTCTG8800.051.747815
AAAAAGG8850.051.0716369
GATCTCG8500.050.32726739
CAGTCAC8950.050.10959627
GCACACG9100.050.04182411
ACACGTC9300.048.96565613
CACACGT9300.048.96565612
TCCAGTC9300.047.85280225
CACGTCT9550.047.6838314
AGCACAC9650.047.547210
Produced by FastQC (version 0.11.2)