FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_c0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_c0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9544437
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140310.14700709952823826No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA106250.1113213906697692TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG20100.035.53006748
CGTATGC20800.034.16847646
GTATGCC26350.027.2335647
ATGCCGT27950.025.67457449
CGCGCGT11600.025.5816291
TCGTATG27950.025.42770245
CTCGTAT30950.023.07445144
TATCTCG31350.022.77944641
TGCCGTC35250.020.55331650
CGCGTAT10150.019.3774471
CGCGCCT17650.018.376841
GGGCGCT5150.018.0902371
GCCGTCT41250.017.81418851
TCTCGTA42000.017.08537143
CCTTTAT42650.016.66320637
GGCGGGC11900.016.52487813
CGGCAAT26500.016.4063661
CGCGGTT17450.016.2145961
GCGCCTG19700.016.1127012
CGGGCAT17350.015.9102951
Produced by FastQC (version 0.11.2)