FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8020709
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250850.31275290002417494No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG172180.21466930167894135RNA PCR Primer, Index 26 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC27050.046.8084844
TATGCCG28900.044.0505746
GTATGCC31650.040.44112445
ATGCCGT31600.039.8500447
TCGTATG32200.039.10627743
CTCGTAT35600.034.88686842
TGCCGTC39400.032.04831348
GCCGTCT41250.030.6946349
CGCGCGT13650.028.3100661
CATCTCG44600.027.38280339
TCTCGTA46000.026.77440641
TCGGGCG21900.025.6768727
GCACACG51700.025.2905411
ACGTCTG52900.024.7166915
CACATGA54250.024.29285231
CACACGT54550.024.09570912
ACACGTC55550.023.66194213
GTCACAT56700.023.18318429
GCATCTC54000.022.9994938
CACGTCT57850.022.78082714
Produced by FastQC (version 0.11.2)