FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658596
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23600.036.10813546
TATGCCG24050.035.00248
TCGTATG31100.027.5113245
ATGCCGT31300.027.33578749
GTATGCC31550.027.22827347
CGCGCGT13550.024.6997931
CGCGTAT11150.024.1368521
CTCGTAT37200.023.0002744
TGCCGTC38750.022.2582250
TCGGGCG16100.022.0707577
CGGGCGC15850.021.3306815
TATCTCG41700.020.5173641
CGCGCCT24350.020.4044271
GGCGGGC17050.020.23408313
GCGGGCG16900.020.00540214
ATCGGGC19750.019.7389166
CGGGCAT19200.019.0487081
GCCGTCT46700.018.76414551
GGGCGGC20100.017.3353739
TCTCGTA50650.016.96023643
Produced by FastQC (version 0.11.2)