FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111479
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26600.030.09084944
TATGCCG27950.028.26695846
GTGTGCG36150.026.3394878
GTATGCC34150.023.43811245
GCCAGTA53000.023.0453051
CGGGCGC31900.022.81937215
GGCGGGC32100.022.5696513
TCGTATG36500.022.30696743
ATGCCGT36000.022.04198547
GTGCGAG44750.021.35488710
GCGGGCG36300.020.05332614
CTCGTAT40250.019.79967342
TGTGCGA49250.019.6837549
TAGCCCG37600.019.36035334
CGCTATG48750.019.2493929
ACCCGTA50000.019.1818632
GCGAGTC51600.018.98799912
TGCGAGT51150.018.8852611
TGTGTGC53800.018.7243637
CGCGTAT17950.018.6449971
Produced by FastQC (version 0.11.2)