FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16380697
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23850.023.28981446
GCCAGTA56150.023.2276361
TATGCCG24850.022.49122848
GTGTGCG39200.022.001558
CGGGCGC31450.019.96466415
GGCGGGC33100.019.28218813
GTGCGAG47450.018.32211510
TAGCCCG35300.017.88522134
CGGCAAT51150.017.4035191
GTATGCC32500.017.30345247
TGCGAGT49950.017.1978911
TGTGCGA51000.017.1817829
AGTAGTC74100.016.9939334
ATGCCGT33050.016.91095249
CGCGTAT21250.016.8864141
TCGTATG33250.016.80938745
CAGTAGT76900.016.509863
TGTGTGC56100.016.418997
ACCCGTA53750.016.36724932
TAGTCAT78400.016.1494336
Produced by FastQC (version 0.11.2)