FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800019
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG30100.026.4756588
CGTACGT19100.025.10704634
GTACGTA19400.025.07371535
TATGCCG19000.025.05814248
CGTATGC19250.024.55359346
CGGGCGC24600.022.15800515
GTGCGAG36050.022.1063610
GCCAGTA35850.021.848831
TACGTAA23250.021.07028436
GGCGGGC26000.020.83228113
CCGTACG23250.020.7739633
TGTGCGA40250.019.8850699
TGCGAGT40500.019.84775511
ATGCCGT24400.019.79527749
TGTGTGC43850.019.7471127
GCGAGTC41950.019.57291412
GTATGCC25250.019.40225447
TCGTATG25600.019.27175745
ACCCGTA41600.019.15723632
TAGCCCG29650.018.73334534
Produced by FastQC (version 0.11.2)