FastQCFastQC Report
Tue 13 Mar 2018
HLFNHBGX5_n01_b5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLFNHBGX5_n01_b5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12271368
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20300.026.00256346
TATGCCG23350.023.04915248
GTATGCC25750.021.16901447
CGCGTAT12200.019.5144391
GTGTGCG22800.019.3676288
ATGCCGT28400.018.95077949
TCGTATG29600.018.41561345
GCCAGTA33900.016.8956621
CTCGTAT32250.016.04690244
CGGGCGC13000.015.922749515
ACCCGTA28050.015.86624232
AGTCACG35600.015.50586728
GTGCGAG28250.015.14317510
CGTTTCG37550.014.51658333
TGGCGGG31400.014.1736857
GTCACGT37650.014.02014629
GCACACG39700.013.99101111
CCCTCGC30650.013.95798166
CCCGTAA32150.013.95017433
TGCCGTC41100.013.934360550
Produced by FastQC (version 0.11.2)